B-10230

Na2HPO4 x 2 H2O - 7.9 KH2PO4 - 1.5 NH4CL - 0.3 MgSO4 x H2O - 0.1 Nrace element solution SL-10 - 1.0 ml Yeast extract - 1.0 Agar - 20.0 Dist. water - 1000.0 ml pH 7.5 - 8.0 Nrace element solution SL-10 HCL(25% 7.7 M) - 10.00 ml FeCl2 x 4 H2O - 1.50 g ZnCl2 - 70.00 mg MnCL2 x 4 H2O - 100.00 mg H3BO3 - 6.00 mg CoCl2 x 6 H2O - 190.00 mg CuCl2 x 2 H2O - 2.00 mg NiCl2 x 6 H2O - 24.00 mg Na2MoO4 x 2 H2O - 36.00 mg Dist. water - 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.